Expression of the protective epitope 190/4 at the cell surfaces of dog cell transfectants. Expression of the 190/4 and M2 epitopes at the cell surfaces of AGMK (A), cr5 (B), flag (C), mut1 (D), mut2 (E), mut3 (F), d1− (G), and DR2 (H) cells was determined by ELISA with twofold dilutions of MAb 190/4 (circles) or M2 (triangles). Absorbance at 450 nm (y axis) was ploted versus the log10 of the MAb dilution (x axis). Data are means of results for duplicate wells; duplicate values varied by less than 10%. The results are from one experiment which was repeated at least two times, with an experimental error of approximately 5 to 10%.