FIG. 8.

MAb 190/4-mediated binding inhibition of HAV to dog cell transfectants. Monolayers of AGMK, cr5, flag, and DR2 cells grown in 96-well plates were treated with 20 μg of MAb 190/4 per ml or mock treated, infected with purified HAV HM175 for 1 h, and washed extensively. HAV bound to the cells was detected with ¹²⁵I-labeled human anti-HAV Ab and autoradiography. The binding inhibition was calculated by densitometric analysis of the autoradiography results as percentages calculated from the integrated OD remaining after monolayers were treated with protective MAb 190/4 compared to those of untreated monolayers. The results are from one experiment which was repeated three times, with an experimental error of approximately 5 to 10%.