TABLE 1.

Oligonucleotides used in this study

Namea	Sequenceb
cr196-218+	ATG CAT CTT CAA GTG GTC ATC TT
mut1+	AGC TTT TGG GGA ACC TTT CAC GCA GGG ATG TCT CTT TGA CTA TAG CAA ATA CAG
mut1−	CTG TAT TTG CTA TAG TCA AAG AGA CAT CCC TGC GTG AAA GGT TCC CCA AA
mut2−	ACT GTC AGA CAC AGC TGT ATT TGC TAT AGT CAA AGA GAC ATC CCT GCG TGA AAG GACPvuII CCC CAA AAG CTT ATA GCG TGT CTC CTT CCG ATA GGT GAC GTG GGT TCC ATT GGT CCA HindIII GAC AAT GCC ATC TGG GCA TGA GAA AAC AGA ACA TGT GCC TC
mut3−	GAC CCC CAA AAG CTT ATA GCG TGT CTC CTT CCG ATA GGT GAC GTG GGT TCC TTG GGT HindIII CCA GAC AAT GCC AT
crfD1+	TGT CAG TTC TTA AGC CTC ATC CTA CAT CTG GCA GAT TCT GTA GCC GAT TCT GTA AAT AflII GTT GAT GGA GTG GCA GGT ACC CCA CCC
crfD1−	GCT AGT TCG AAC AGT TCT GAC AAT TGG AGT AGT GAC TCT GGG TGG GGT ACC TGC CAC TCC BstBI
HAVcr-flag1+	CAT GGA CTA CAA GGA CGA CGA TGA CAA GCC CGG GTT SmaI
HAVcr-flag1−	CAT GAA CCC GGG CTT GTC ATC GTC GTC CTT GTA GTC SmaI

^aThe last character of the oligonucleotide name indicates its polarity. −, negative sense; +, positive sense. 

^bMutations are in boldface type. Restriction sites are underlined and named below the line.