Table 1.
Purified AAV titer (vg/mL), purified AAV yield (vg), harvest yield (vg/mL culture), and VP1:VP2:VP3 ratio for AAV lots used in this study
| Experiment | Sample | Purified AAV titer (vg/mL) | Purified AAV yield (vg) | Harvest yield (vg/mL culture) | VP1 | VP2 | VP3 |
|---|---|---|---|---|---|---|---|
| 1 | Bac-RepCap | 2.23E+13 | 2.45E+13 | 1.23E+11 | 1 | 2.1 | 45 |
| SGMO Helper | 5.99E+13 | 8.99E+13 | 4.50E+11 | 1 | 1.1 | 21 | |
| 2 | Bac-RepCap | 2.25E+13 | 2.70E+13 | 1.35E+11 | 1 | 2.1 | 60 |
| SGMO Helper | 7.97E+13 | 1.20E+14 | 6.00E+11 | 1 | 1.3 | 24 | |
| 3 | Bac-RepCap | 9.46E+12 | 9.46E+12 | 4.73E+10 | 1 | 2.1 | 67 |
| SGMO Helper | 6.21E+13 | 8.69E+13 | 4.35E+11 | 1 | 1 | 19 | |
| 4 | Bac-RepCap | 1.83E+13 | 1.65E+13 | 8.25E+10 | 1 | 1.8 | 60 |
| SGMO Helper | 8.40E+13 | 1.09E+14 | 5.45E+11 | 1 | 0.8 | 17 | |
| Serial passaging | SGMO Helper P.0 | 1.84E+13 | 1.29E+13 | 6.45E+10 | 1 | 0.4 | 16 |
| SGMO Helper P.3 | 1.05E+12 | 9.45E+11 | 4.73E+09 | 1 | 0.8 | 18 | |
| SGMO Helper P.6 | 2.70E+12 | 3.24E+12 | 1.62E+10 | 1 | 0.4 | 17 | |
| Mid-scale production | Bac-RepCap | 3.56E+13 | 3.98E+14 | 2.65E+11 | 1 | 1.7 | 82 |
| SGMO Helper | 4.85E+13 | 9.89E+14 | 6.59E+11 | 1 | 1.2 | 24 | |
| Mid-scale production for long-read sequencing | Bac-RepCap | 9.89E+13 | 8.41E+14 | 5.61E+11 | 1 | 1.8 | 99 |
| Bac-RepCap | 5.31E+13 | 3.98E+14 | 2.65E+11 | 1 | 1.4 | 88 | |
| SGMO Helper | 2.75E+14 | 3.38E+15 | 2.25E+12 | 1 | 0.7 | 34 | |
| SGMO Helper | 1.02E+14 | 9.89E+14 | 6.59E+11 | 1 | 1.0 | 24 | |
| In vivo study | Bac-RepCap | 1.37E+13 | 3.01E+13 | 1.51E+11 | 1 | 1.5 | 59 |
| SGMO Helper | 3.47E+13 | 1.15E+14 | 5.75E+11 | 1 | 0.9 | 27 |