Table 4.
Quantitation of ITR content in sequencing reads that map to the vector genome
| Sample | Total mapped reads | Both ITR reads (% total reads) | Left ITR-only reads (% total reads) | Right ITR-only reads (% total reads) | No ITR reads (% total reads) | ITR-only reads (% total reads) |
|---|---|---|---|---|---|---|
| Bac-RepCap | 146,721 | 52,634 (35.9%) | 41,721 (28.4%) | 45,138 (30.8%) | 5,024 (3.4%) | 453 (0.3%) |
| Bac-RepCap | 187,604 | 69,519 (37.1%) | 52,108 (27.8%) | 57,551 (30.7%) | 6,073 (3.2%) | 462 (0.2%) |
| SGMO Helper | 205,347 | 64,478 (31.4%) | 55,963 (27.3%) | 75,853 (36.9%) | 4,185 (2.0%) | 508 (0.2%) |
| SGMO Helper | 152,342 | 53,898 (35.4%) | 37,727 (24.8%) | 51,672 (33.9%) | 5,838 (3.8%) | 368 (0.2%) |
| Significant? (t test, alpha = 0.05) | No (p = 0.867) | No (p = 0.995) | No (p = 0.457) | No (p = 0.638) | No (p = 0.807) |
Values are presented as read count or percentage of the total reads used in this analysis; t tests were used to compare each category of ITR content for each sample.