Table 5.
Mapping of long-read sequencing truncations to the vector genome
| Nucleotide position (1–2,772) | Bac-RepCap | Bac-RepCap | SGMO Helper | SGMO Helper | Significant? (t test) |
|---|---|---|---|---|---|
| 2,772 | 107,421 (100%) | 137,442 (100%) | 173,535 (100%) | 124,655 (100%) | No (p = 0.451) |
| 2,677 | 76,927 (35%) | 106,331 (36%) | 121,382 (35%) | 94,742 (36%) | No (p = 0.495) |
| 1 | 68,183 (100%) | 88,652 (100%) | 91,370 (100%) | 68,655 (100%) | No (p = 0.926) |
| 74 | 59,316 (29%) | 79,540 (30%) | 81,865 (30%) | 62,415 (30%) | No (p = 0.865) |
| 46 | 29,130 (20%) | 38,902 (21%) | 38,299 (21%) | 31,084 (21%) | No (p = 0.922) |
| 27 | 28,626 (29%) | 34,801 (28%) | 34,849 (27%) | 28,627 (29%) | No (p = 0.996) |
The top six truncations identified by long-read sequencing are included and the frequency of each truncation is reported. The percentage of truncations vs. total reads at each position is reported in parentheses; t tests were used to compare the frequency of truncations in each sample.