Table 1.
Comparative table of methods
| Current protocol | SCENITH | Seahorse | Mass spectrometry | |
|---|---|---|---|---|
| Consumable cost/samplea | Low (< 1 USD) | Medium (1–5 USD) | High (≈10 USD) | High (5–10 USD) |
| Instrumentation cost | Low | High | High | Very high |
| Technical expertise required | Less | High | High | Very high |
| High throughput amenability | Very high | Low | High | High |
| Read out for metabolic profiling | Luminescence-based ATP | Protein synthesis rate as a surrogate marker for ATP | Extracellular oxygen, and pH | Metabolites |
| Single cell resolution | No | Yes | No | Yesb |
| Time (Hrs.) for profiling a batch of 24 samples.c | 4 | 4–65 | 242 | 1–24d |
a
Estimated cost excluding cell culture. Costs are empirical, may differ according to the brands used.
b
Single-cell metabolomics platforms. e.g.- MALDI, SIMS, DESI etc.6
c
Time calculated from introduction of metabolic inhibitor to data acquisition.
d
Depending upon the platform (e.g., – MALDI, LC-MS, GC-MS etc.), run time of each sample is 5–60 min.