REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Dulbecco’s modified Eagle’s medium (DMEM)	HiMedia	AT006
Penicillin G sodium salt	HiMedia	TC020
Streptomycin sulphate	HiMedia	TC035
Fetal bovine serum, certified, United States	Gibco	16000044
Trypsin-EDTA (0.25%), phenol red	Gibco	25200072
2-deoxy-D-glucose	TCI	D0051
DMSO	Sigma-Aldrich	D8418-100ML
Oligomycin A	Sigma-Aldrich	75351
1,1-dimethylbiguanide hydrochloride (metformin)	Sigma-Aldrich	D150959
Trypan blue solution, 0.4%	Gibco	15250061
Sodium chloride	Sigma-Aldrich	S9625
Potassium chloride	Sigma-Aldrich	P9541
Sodium phosphate dibasic	Sigma-Aldrich	S9763
Potassium phosphate monobasic	Sigma-Aldrich	P0662
Hydrochloric acid	Sigma-Aldrich	258148
Critical commercial assays
Cell proliferation kit II (XTT)	Sigma-Aldrich	11465015001
Luminescent ATP detection assay kit	Abcam	ab113849
Experimental models: Cell lines
HepG2 cell line	Mukherjee et al.13	NA
Software and algorithms
Microsoft Office	Microsoft	www.microsoft.com
GraphPad Prism 9.0	Dotmatics	https://www.graphpad.com
BioRender	BioRender	https://www.biorender.com
Other
100 mm cell culture dishes	Thermo Fisher Scientific	150464
Nunc serological pipettes	Thermo Fisher Scientific	170356N
Microscopic cover glass	Blue Star	https://bluestarslides.com
Eppendorf conical tubes 15 mL	Eppendorf	0030122151
Counting chamber BLAUBRAND Neubauer improved, w/o clips double ruling	BRAND	717805
Nunc 96-well flat bottom, clear	Thermo Fisher Scientific	161093
Nunc 96-well flat bottom, white	Thermo Fisher Scientific	136102
BioTek Synergy HTX multimode reader	BioTek	1623207