REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Acetic acid, 99.7%, ACS reagent	Sigma-Aldrich	Cat# 00761AJ
Agar	Sigma-Aldrich	Cat# A7002-250G
Chicago Sky Blue 6B	Sigma-Aldrich	Cat# 8679
DAPI Fluoromount-G	Southern Biotech	Cat# 0100-20
DPX mountant for histology	Sigma-Aldrich	Cat#06522-100ML
Ethanol	Panreac Química S.L.U.	Cat# 131086.1214
Fluoromount-G	Southern Biotech	Cat# 0100-01
Formalin (4% formaldehyde)	Sigma-Aldrich	Cat# 1.00496
Gelatin powder	Merck	Cat# 1.04078.1000
Heparin	Laboratorio Reig Jofré, S.A.	Cat# 608737.4
Hoechst 33342 dye	Sigma-Aldrich	Cat# B2261
Isoflurane (AErrane)	Baxter	Cat# FDG9623
Lidocaine 2%	Normon	Cat# P06B1
Methanol	Sigma-Aldrich	Cat# 34860-1L-R
Pentobarbital sodium (Dolethal)	Vetoquinol Group	N/A
Phosphate buffer solution 0.1 M (PB)	Sigma-Aldrich	Cat# P5244
Phosphate-buffered saline (PBS)	Sigma-Aldrich	Cat# P7059
Povidone iodine 10%	Laboratorio Reig Jofré, S.A.	Cat# 838151.7
Saline solution 0.9%	B. Braun Medical, S.A.	Cat# 607189.2
Sucrose	Sigma-Aldrich	Cat# S9378
Thionine acetate salt	Sigma-Aldrich	Cat# T7029-5G
Tissue freezing medium	Leica Biosystems	Cat#14020108926
Xylene	Sigma-Aldrich	Cat# 534056
Experimental models: Organisms/strains
C57BL6/J male or female mice (8–16 weeks)	Jackson Laboratory	JAX:000664, RRID:IMSR_JAX:000664
Software and algorithms
Igor Pro, Wavemetrics	WaveMetrics	RRID:SCR_000325 https://www.wavemetrics.com
ImageJ	Schneider et al.6	RRID:SCR_003070 https://imagej.nih.gov/ij/
MATLAB	The MathWorks, Inc.	RRID:SCR_001622 https://jp.mathworks.com/
OmniPlex Server and PlexControl software	Plexon Inc	RRID:SCR_014803
SARFIA (Igor Pro, Wavemetrics)	Dorostkar et al.7	https://www.wavemetrics.com/project/SARFIA
Spike2 v7	Cambridge Electronic Design (CED)	RRID:SCR_000903
Deposited data
Custom-written scripts for processing data in Spike2	This study	https://doi.org/10.5281/zenodo.10365406
Other
8-channel digital headstages	Omnetics Connector Corporation	HST/8o25-9P-GR
Adapter for recording probe (32–16 channels)	NeuroNexus Technologies Inc.	Adpt-A16-A32
Alloy thread of tin (60%) and lead (40%)	Multicore	Cat# 295-4836
Anesthesia system	Medical Supplies & Services, Int. Ltd	N/A
Banana connector	RS Amidata S.A.U.	N/A
BNC cable	RS Amidata S.A.U.	Cat#426-2038
Bulldog clamp	F.S.T	Cat# 18039-45
Cranial screws M1 x 4 mm	F.S.T.	Cat# 39886
Cryostat CM1520	Leica Biosystems	N/A
Delicate suture tying forceps	F.S.T	Cat# 11063-07
Digital stimulator DS8000	World Precision Instruments	RRID:SCR_008593
Drill	F.S.T	Cat# 097034
Drill bit	F.S.T	Cat# 19007-05
Dumont #7 forceps	F.S.T	Cat# 91197-00
Gelfoam sponge	Ferrosan Medical Device	Cat# 4547333
Fine scissors-sharp	F.S.T	Cat# 14060-10
Hair clipper	Aesculap Inc.	Cat# GT606
Heat shrink tubing	RS Amidata S.A.U.	Cat# 481-1775
Homoeothermic blanket	Cibertec SL	Cat# RTC-1
Hypoallergenic non-wave tape CPK TAPE	Farmaban	Cat# 1436
Leukosan adhesive	Leukoplast	Cat# 730-585
Linear vertical array of 32 iridium contact	NeuroNexus Technologies Inc.	A1X32-6mm-50-177-A32
Linear vertical arrays (A1x32) adaptor	Samtec	MOLC-110-01-S-Q
Lubrithal eye gel	Dechra	N/A
Microscope TCS SP5	Leica Microsystems	N/A
Microtome Microm HM450	Thermo Fisher Scientific	N/A
Multicore copper cable	RS Amidata S.A.U.	Cat# 285-7713
Multimeter	Amprobe	Cat# 5XP/A
OmniPlex System	Plexon	RRID:SCR_014803
One-axis oil hydraulic micromanipulator	Narishige International Limited	Cat# MO-10
Plastic pipette	VWR	Cat# 612-2846P
Scalpel	Swann-Morton	Cat# 0305
Soldering station (24 V, SMT unit 60 A)	ERSA	N/A
Stimulus isolator unit	ISO-Flex, A.M.P.I.	RRID:SCR_018945
Stereotaxic frame and ear bar for mice	Narishige International Limited	Cat# SR-6M-HT and Cat# EB-5N
Stereotaxic micromanipulator	Narishige International Limited	Cat# SM-15R/L
Surgical microscope M300 and light source CLS 150 MR	Leica Microsystems	N/A
Surgical caliper	F.S.T	Cat# 18000-35
Syringe 0.5 mL	B. Braun Medical, S.A.	Cat# 9161619S
Tissue multiwell plate	VWR	Cat# 734-2779
Tweezers	F.S.T	Cat# 11008-13
Universal pliers	RS Amidata S.A.U.	Cat# 161-164 and Cat# 221-4946
Vaseline	Laboratorio Reig Jofré, S.A.	Cat# 845008-EFP
Vibratome VT1000S	Leica Biosystems	RRID:SCR_016495