Figure 6.

Mild hypothermia of 35℃ promoted transplanted hNSCs differentiation into neurons in vivo

(A) The timeline of the hNSCs transplantation into mouse striatum with normothermia or mild hypothermia treatments.

(B–E) Representative immunofluorescence images of β-tubulin III, DCX, S100β, and GFAP positive GFP-labeled cells at Day 14 with normothermia or mild hypothermia treatments.

(F) The colocalization coefficient of β-tubulin III, DCX, MAP2, S100β, and GFAP positive GFP-labeled cells in (B–E) and Figure S6E. All data presented as mean ± SD. Student’s t tests were used in (F). ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Scale bars represent 50 μm in (B–E).

See also Figure S6.