REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
β-Tubulin III	Abcam	Cat# ab78078; RRID: AB_2256751
β-Tubulin III	Abcam	Cat# ab52623; RRID: AB_869991
β-Tubulin III	Sigma	Cat# T8660; RRID: AB_477590
DCX	Abcam	Cat# ab18723; RRID: AB_732011
S100β	Abcam	Cat# ab52642; RRID: AB_882426
GFAP	Cell Signaling Technology	Cat# 3670; RRID: AB_561049
GFAP	Cell Signaling Technology	Cat# 12389; RRID: AB_2631098
SOX2	Cell Signaling Technology	Cat# 3579s; RRID: AB_2195767
RBM3	Proteintech	Cat# 14363-1-AP; RRID: AB_2269266
SOX11	Millipore	Cat# HPA000536; RRID: AB_1080060
SOX11	Abmart	Cat# T55823
β-actin	Cell Signaling Technology	Cat# 4970; RRID: AB_2223172
GFP	Abcam	Cat# ab13970; RRID: AB_300798
V5	Abmart	Cat# M20052
Flag	Abways	Cat# AB0008; RRID: AB_2943672
MAP2	Abcam	Cat# ab5392; RRID: AB_2138153
Anti-Rabbit 488	Cell Signaling Technology	Cat# 4412; RRID: AB_1904025
Anti-Rabbit 594	Cell Signaling Technology	Cat# 8889; RRID: AB_2716249
Anti-Mouse 488	Cell Signaling Technology	Cat# 4408; RRID: AB_10694704
Anti-Mouse 594	Cell Signaling Technology	Cat# 8890; RRID: AB_2714182
Anti-Chicken 647 IgY	Invitrogen	Cat# A-21449; RRID: AB_2535866
HRP-linked anti-Rabbit IgG (H + L)	Beyotime	Cat# A0208; RRID: AB_2892644
HRP-linked anti-Mouse IgG (H + L)	Beyotime	Cat# A0216; RRID: AB_2860575
HRP-linked anti-Chicken IgG (H + L)	Abcam	Cat# ab6877; RRID: AB_955465
Bacterial and virus strains
DH5α	Shanghai Sangon Biotechnology Co., Ltd	B528413
pLenti-EF1-EGFP-P2A-Puro	OBIO Technology (Shanghai) Co., Ltd	N/A
Chemicals, peptides, and recombinant proteins
Basic fibroblast growth factor	Gibco	PHG0367
Epidermal growth factor	Proteintech	HZ-1326
Critical commercial assays
DMEM/F12	Gibco	10565018
B27 supplement	Gibco	17504044
Accutase	Gibco	A1110501
Lipofectamine 2000	Invitrogen	11668019
neurobasal medium	Gibco	21103049
L-GlutaMax	Gibco	35050079
polybrene	Biosharp	Bl628A
4-Thiouridine	Sigma	T4509
DTT	ThermoFisher	R0862
RNase inhibitor	Sangon Biotech	B600478
EDTA-Free Protease Inhibitor Cocktail	Bimake	B14001
TRIzol	Vazyme	R401-01
RNase 1	Thermo Scientific	EN0601
Protease K	Sangon Biotech	A610451
RIPA buffer	Beyotime	P0013B
SDS-PAGE Gel	Vazyme	E304/305
BCA protein assay kit	Sangon	C503021
ECL	Millipore	WBULS0500
HiScript III 1st Strand cDNA Synthesis Kit	Vazyme	R312-01
ChamQ Universal SYBR qRT-PCR Master Mix	Vazyme	Q711-02
Actinomycin D	MedChemExpress	HY-17559
EdU assays kit	EpiZyme	CX004
Dual-Luciferase Reporter Assay Kit	Yeasen	11402ES60
DAPI	Ysasen	40727ES10
Deposited data
Single-cell RNA sequencing data	This paper	GEO: GSE241889
CLIP-RNA sequencing data	This paper	GEO: GSE241548
Original, unprocessed data	This paper	Mendeley Data: https://doi.org/10.17632/7527hjwbnv.1
Experimental models: Cell lines
Human neural stem cells	Shanghai Angecon Biotechnology Co., Ltd	N/A
HEK293T	Cell Bank/Stem Cell Bank, Chinese Academy of Sciences (Shanghai, China)	CSTR:19375.09.3101HUMSCSP5209
Experimental models: Organisms/strains
BALB/C nude mice	Experimental Animal Center of Ren Ji Hospital	N/A
Oligonucleotides
siRNA oligo sequence	This study, see Table S3	N/A
Primers for qRT-PCR	This study, see Table S7	N/A
Recombinant DNA
pMS2	Shanghai Jiao Tong University	N/A
pMS2-GST	Shanghai Jiao Tong University	N/A
pmirGLO	Beijing Tsingke Biotechnology Co., Ltd	N/A
pCDH-CMV-RBM3-EF1-copGFP	Beijing Tsingke Biotechnology Co., Ltd	N/A
pLVX-RBM3-3Flag-Puro	Beijing Tsingke Biotechnology Co., Ltd	N/A
pCDH-CMV-RBM3mut-EF1-copGFP	Beijing Tsingke Biotechnology Co., Ltd	N/A
pLVX-RBM3mut-3Flag-Puro	Beijing Tsingke Biotechnology Co., Ltd	N/A
pLX304-CMV-RBM3-V5	Shanghai Jiao Tong University	N/A
pCDH-CMV-SOX11-EF1-copGFP	Beijing Tsingke Biotechnology Co., Ltd	N/A
pLVX-SOX11-3Flag-Puro	Beijing Tsingke Biotechnology Co., Ltd	N/A
Software and algorithms
Prism 8	GraphPad	https://www.graphpad.com/
ImageJ	ImageJ	https://imagej.net/software/imagej/
catRAPID omics v2.0	catRAPID	http://service.tartaglialab.com/page/catrapid_omics2_group
Biorender	Biorender	https://biorender.com/
Other
Temperature-controlled blanket	CWE Inc	TC-1000
Stereotactic apparatus	RWD	N/A
Micro syringe pump	Harvard Apparatus	Pump 11 Elite