FIG. 10.

The activity of core particle-associated kinase is resistant to proteinase K digestion. Purified intracellular core particles (A and B) and solubilized PKA (C and D) were treated with increasing concentrations of proteinase K (1, 5, 25, 50, 100, and 200 ng) and assayed by incorporation of [γ-³²P]ATP into core protein (A) and autophosphorylation of PKA (C) or by an in-gel kinase assay with GST-2/5C as the substrate, as described in the legend to Fig. 7 (B and D).