Table 2.
Kinetics of development at 48 and 96 h post-insemination (hpi) and cumulative blastocyst rates on Days 7 and 8 after IVC with or without miR-148b mimic or control mimics
| IVC N |
Total cleaved N (%±SEM) |
< 16 cells N (%±SEM) |
≥ 16 cells N (%±SEM) |
IVC 96 hpi |
Blastocysts | ||
|---|---|---|---|---|---|---|---|
| D7 | D8 | ||||||
| N (%±SEM) |
N (%±SEM) |
||||||
| Control | 1001 | 874 (87.3 ± 0.7) | 230 (23.2 ± 0.6) | 644 (64.1 ± 0.7) | 881 | 212 (24.4 ± 0.5) | 274 (31.2 ± 0.6) |
| CMimic | 1011 | 878 (86.9 ± 0.6) | 229 (22.7 ± 0.4) | 649 (64.2 ± 0.7) | 891 | 212 (23.8 ± 0.5) | 272 (30.6 ± 0.8) |
| miR148b | 1019 | 891 (87.5 ± 0.4) | 228 (22.5 ± 0.5) | 663 (64.9 ± 0.6) | 899 | 217 (24.2 ± 0.6) | 282 (31.4 ± 0.8) |
| miR148b-OV | 1052 | 917 (87.7 ± 0.5) | 253 (23.1 ± 0.4) | 664 (64.5 ± 0.5) | 932 | 223 (24.4 ± 0.5) | 290 (31.0 ± 0.5) |
| miR148b-UT | 1056 | 927 (87.4 ± 0.5) | 245 (23.3 ± 0.6) | 682 (63.1 ± 0.4) | 936 | 228 (23.9 ± 0.6) | 291 (31.1 ± 0.8) |
Embryos cultured from presumptive zygotes to blastocyst in SOF + 0.3% BSA (Control), SOF + 0.3% BSA supplemented with 1 µM control mimics (CMimic), or SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b) or embryos cultured from presumptive zygotes to 16- cell stage in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-OV), or embryos cultured from 16-cell stage to BD7 in SOF + 0.3% BSA supplemented with 1 µM of miR-148b (miR148b-UT). Data are the mean ± s.e.m. Within columns, different superscript letters indicate significant difference (P ≤ 0.001) between treatments