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. Author manuscript; available in PMC: 2024 Mar 23.
Published in final edited form as: Acta Biomater. 2021 Oct 30;138:453–462. doi: 10.1016/j.actbio.2021.10.048

Fig. 2.

Fig. 2.

Cellular study of IMQ@IONs/ICG with or without laser irradiation. (A) Representative fluorescence images of Panc02-H7 cells showing intracellular uptake of nanoparticles. Blue: DAPI, Red: Lyso-tracker, Green: ICG. (B) Representative fluorescence images of Panc02-H7 cells showing intracellular production of •OH (green) induced by nanoparticles. Blue: DAPI, Green: APF. (C, D) Representative flow scatter plot and fluorescence images showing cell death of Panc02-H7 cells after treatment with IMQ@IONs/ICG plus laser irradiation (0.75 W cm −2, 5 min), with Annexin V-FITC/PI staining (C) or Calcein-AM/PI staining (D). (E) Representative fluorescence images of Panc02-H7 cells showing CRT exposure on the surface after treatment with IMQ@IONs/ICG plus laser irradiation. (F, G) Representative flow graph showing CD80 and CD86 expressions on the surface of DCs stimulated by different nanoparticles.