Table 2. Reaction composition.

Genotyping PCR			Reverse-transcription reaction
Component	Volume (µL)		Reagent	Volume (µL)
2× Taq PCR mix	5		Reaction solution from Step 24	10
Genomic DNA (100–200 ng/uL)	1		5X PrimeScript Buffer 2 (for real time)	4
Primer pairs (forward and reverse, 2.5 mmol/L)	0.8		PrimeScript RT Enzyme Mix I	1
Distilled water	3.2		RT Primer Mix	1
Total	10		RNase free dH2O	4
			Total	20