Figure 1. Cutaneous wounding using a rotary tool.

(a) Image of cutaneous wounding setup. (b) 1/16th and 1/8th inch square end mill cutting bits used for generating cutaneous wounds. (c) Magnified side and front views of 1/16th inch cutting bit tip. (d) 3D rendering of the plastic printed cowling. (e) Schematic diagram showing attachment of the cowling to the rotary tool. (f) 3D rendering of the plastic printed holding platform with rotating insert. (g) Higher magnification image of the rotary tool with cutting bit, cowling, and holding platform on a dissecting microscope. (h-j) Images of mounting fish onto the holding platform, showing a platform with wetted filter paper (h), a fish placed on the platform on top of the paper (i), and a mounted fish immobilized on the platform with anchoring rods (j).