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. Author manuscript; available in PMC: 2025 Apr 1.
Published in final edited form as: J Invest Dermatol. 2023 Nov 17;144(4):888–897.e6. doi: 10.1016/j.jid.2023.10.015

Figure 4. Neutrophils are rapidly recruited to cutaneous wounds.

Figure 4.

(a-b) Confocal images of adult Tg(lyz:DsRed2)nz50 transgenic casper fish labeling neutrophils (magenta) (a) or 3D rendering of neutrophil spots (magenta) with dragon tails (cyan) from last 15 minutes of movement (b) demonstrating stages of recruitment to the wound. (c) Quantification of neutrophil number in the wound over 17 hours. Black dotted line represents the exponential best fit. (d) Graph showing the mean track speed of neutrophils in uninjured and injured fish. (e) Confocal images and track heatmap of neutrophil clustering in uninjured and injured fish. Bluer tracks indicate closer cells. (f) Graph depicting distance of neutrophils to nine nearest neighbors over time in uninjured and injured fish. Error bars indicate mean +/− SD. Welch’s t-test, ****p<0.0001. Images are oriented anterior left and dorsal up. Scale bars = 400μm (a-b, e).