FIG. 2.
Analysis of HIV-1 infection in the presence of MG132 on various cell lines. HeLa-CD4 cells and the Jurkat, CEM, and HUT78 T-lymphoid cell lines were infected with the HIV-HSA reporter virus pseudotyped with the VSV-G envelope, which contains, in the place of nef, the gene coding for HSA (CD24). Following integration and proviral expression, cells synthesize CD24, which can be detected at the cell surface. Cell lines were infected with HIV-HSA(VSV) (50 ng of p24Gag) for 1 h, with or without MG132 (50 μM). Infection was revealed 24 h later by staining with anti-CD24 monoclonal antibodies and flow cytometry analysis of gated living cells. Data are representative of three independent experiments.