Fig. 4. Rab27A activated the phosphorylation of TYK2.

a, b GSEA based on TCGA data suggested that the high-level expression of Rab27A is related to the activation of the oncogenic pathway, chemokine pathway, JAK-STAT signaling pathway, MAPK signaling pathway, and TGF-β signaling pathway. c Plot map of phosphorylation pathway profiling array indicated the phosphorylation activation of JAK/STAT signaling pathway and AKT signaling pathway. d Relative protein expression level of Rab27A-knockdown cells and negative control cells based on phosphorylation pathway profiling array. e, f Cell lysates from A549 and H1299 cells with the indicated shRNAs were subjected to Western blot analysis to detect the phosphorylation activation of TYK2 (Y1054). g, h Cell lysates from A549 and H1299 cells with the indicated lentiviral vectors were subjected to Western blot analysis to detect the phosphorylation activation of TYK2 (Y1054). i, j After serum starvation for 24 h, A549, and H1299 cells were treated with CM collected from Rab27A-overexpressing cells or negative control cells for 24 h. Cell lysates were subjected to Western blot analysis to detect the phosphorylation activation of TYK2 (Y1054).