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. 2024 Mar 23;8:74. doi: 10.1038/s41698-024-00574-1

Fig. 7. HSPA5 expression is affected by Rab27A and ER stress may be the potential downstream mechanism to mediate malignant biological behavior.

Fig. 7

a Heatmap of differentially expressed protein in A549 and H1299 cells with Rab27A-knockdown. b GO analysis based on differentially expressed protein showed the potential regulated cellular components and biological pathways. c Venn diagram showed the intersection of differentially expressed protein between A549 and H1299 cells. d, e Cell lysates from A549 and H1299 cells infected with the indicated shRNAs or lentiviral vectors were subjected to Western blot analysis to detect the HSPA5 protein level. f, g qRT-PCR analysis of HSPA5 mRNA in A549 and H1299 cells infected with the indicated shRNAs or lentiviral vectors. h Cerdulatinib treatment (1 μM) reversed the promoting effect on HSPA5 mRNA expression induced by Rab27A overexpression. i SiRNAs targeting HSPA5 assay was performed in A549 OE-Rab27A cells for 48 h. Cell lysates were subjected to Western blot analysis to detect the HSPA5 protein level. j The viability of A549 cells overexpressing Rab27A was determined by CCK-8 assay after the knockdown of HSPA5. k–m Rab27A-knockdown inhibited the clonogenic ability, migration ability, and invasion ability of A549 cells overexpressing Rab27A.