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. 2024 Mar 23;14:6971. doi: 10.1038/s41598-024-57090-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining for PGP9.5, GAP43 and tyrosine hydroxylase (TH) in left ventricular myocardium of WT and Nox2 KO mice with saline or Doxorubicin (Dox) treatment. (A) PGP9.5. Bar = 20 μm. (B) The graph shows the relative expression of myocardial PGP9.5 in the four groups. Values are presented as means ± S.E.M.; n = 5–6. *P < 0.05 versus WT saline group. #P < 0.05 versus WT Dox group. (C) GAP43. Bar = 100 μm. (D) The graph shows the relative expression of myocardial GAP43 in the four groups. Values are presented as means ± S.E.M.; n = 5–6. *P < 0.05 versus WT saline group. #P < 0.05 versus WT Dox group. (E) TH. Bar = 20 μm. (F) The graph shows the relative expression of myocardial TH in the four groups. Values are presented as means ± S.E.M.; n = 5–7. *P < 0.05 versus WT saline group. #P < 0.05 versus WT Dox group. Comparisons among groups were performed by one-way ANOVA followed by a Bonferroni post hoc test for multiple comparisons.