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. 2024 Mar 23;14:6958. doi: 10.1038/s41598-024-57286-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of AAV2- and LV-mediated MYOC editing in TM cells. (A) Representative images showing AAV2_crMYOC or LV_crMYOC treatment of TM3 cells stably expressing DsRed tagged mutant MYOC. AAV2_crMYOC or LV_crMYOC reduces intracellular MYOC and ER stress marker GRP78 (cyan) (scale bar = 50 μm; n = 3). (B) Quantitative analysis of fluorescent intensities demonstrates a significant reduction of MYOC fluorescence in both LV and AAV2-crMYOC treated TM cells. For GRP78 immunostaining, only LV_crMYOC cells showed significant decrease. Unpaired (two-tailed) student t test, with *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Quantitative data represented as mean ± SEM.