Table 2.
Primary antibody details
| Target | Host | Dilution | Retrieval/fixation | Supplier | Cat.# | Use |
|---|---|---|---|---|---|---|
|
CD3 (F7.2.38) |
Mouse | 1:50 | None | Dako | M7254 | human T cells (ICC) |
| CD206 | Mouse | 1:50 | Acetone | Biolegend | 321,116 | IHC FF |
| CLDN5 | Rabbit | 1:200 | None | Invitrogen | #34–1600 | hCMEC/D3 |
| Collagen IV | Rabbit | 1:200 | Acetone | Abcam | ab6581 | IHC FF |
| GAPDH | Goat | 1:500 | None | Santa Cruz | SC-20357 | WB hCMEC/D3 |
| HLA-DR (LN3) | Mouse | 1:500 | Citrate, Tris, Acetone | In house | IHC FFPE, FF | |
| IBA1 | Goat | 1:500 | Citrate,Tris | Abcam | AB5076 | IHC FFPE |
| P2RY12 | Rabbit | 1:100 | Citrate,Tris | Atlas antibodies | HPA014518 | IHC FFPE |
| P65 | Rabbit | 1:500 | PFA (4%) | Abcam | ab16502 | ICC hCMEC/D3 |
| Phalloidin | 1:100–250 | None | Molecular Probes | A12379, R415 | ICC hCMEC/D3 | |
| TNFα | Rabbit | 1:200 | Acetone | Origene | PP1071P1 | IHC FF |
| TRPV4 intrac | Rabbit | 1:50 | Acetone | Alomone Labs | ACC034 | IHC FF,WB |
| TRPV4 extrac | Rabbit | 1:50 | Acetone | Alomone Labs | ACC124 | IHC FF |
| TRPV4 | Rabbit | 1:100 | Acetone | Invitrogen | # PA5-41066 | IHC FF |
| UEA-1 | 1:1000 | Acetone | Vector labs | B-1065 | IHC FF |
Citrate pH 6.0; Tris 10 mM Tris / 1 mM EDTA, pH 9.0