TABLE 2.

Percentages and quantities of virus-specific and total antibodies detected by fragment culture at the time of and 4 days after challenge in mice immunized previously with RRV or RRV in SA^a

Postinoculation time and dose	Microcapsule	Mean % of VS IgA ± SEM (VS/total quantities)		Mean % of VS IgG ± SEM (VS/total quantities)
		At challenge	4 days postchallenge	At challenge	4 days postchallenge
6 wks
2.2 × 107	SA	0 (0/2,835)	0.70 ± 0.16 (16/2,283)	0 (0/16)	0 (0/17)
2.2 × 107		0.11 ± 0.01 (4/3,455)	1.07 ± 0.37 (41/3,842)	0 (0/14)	14.8 ± 5.20 (4/27)
6.0 × 106	SA	0 (0/3,444)	0 (0/2,900)	0 (0/19)	0 (0/33)
6.0 × 106		0 (0/4,508)	1.94 ± 0.67 (66/3,405)	0 (0/25)	9.43 ± 1.44 (5/53)
dH2O		0 (0/3,826)	0 (0/3,410)	0 (0/24)	0 (0/19)
16 wks
2.2 × 107	SA	0 (0/1,863)	20.3 ± 5.26 (384/1,896)	0 (0/23)	21.7 ± 8.60 (47/217)
2.2 × 107		0.1 ± 0.03 (3/2,961)	2.0 ± 0.80 (37/1,848)	17.0 ± 5.19 (8/47)	28.3 ± 9.00 (13/46)
6.0 × 106	SA	0.1 ± 0.02 (2/1,921)	1.37 ± 0.40 (30/2,197)	0 (0/23)	1.7 ± 0.82 (1/58)
6.0 × 106		0 (0/3,582)	0 (0/1,503)	0 (0/168)	0 (0/134)
dH2O		0 (0/1,523)	0 (0/1,697)	0 (0/35)	0 (0/57)

^aGroups of mice were orally inoculated with either RRV or RRV in SA microcapsules at various doses. At 6 or 16 weeks after inoculation, the mice were challenged with EDIM. Small intestinal fragment cultures were performed at the time of challenge and 4 days after challenge. Supernatant fluids from fragment cultures were tested for the presence of virus-specific (VS) and total IgA and IgG by ELISA. Virus-specific IgA and IgG were not detectable at quantities of less than 1 ng/ml. Undetectable quantities are shown as 0. Quantities of virus-specific and total antibodies are expressed in micrograms per milliliter. Standard errors of the mean are based on relative quantities of virus-specific and total antibodies from 16 samples.