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. 2024 Mar 23;14:38. doi: 10.1186/s13578-024-01206-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Bacterial-mediated RNAi of c-Myc in colorectal and breast cancer cell lines. (A-C) Immunofluorescence staining demonstrating intracellular infection of cell lines SW480, HCT116 and MCF7 (Red-B-actin; Blue-DAPI & green-bacteria). (D-F) Immunofluorescence staining demonstrating nuclear specific reduction of c-Myc protein at 24 h, 48 and 72 h following infection with SL7207/c-Myc strains in comparison to uninfected control and SL7207/SCR (D-SW480, E- HCT116 & F-MCF7). (G-I) qRT-PCR analysis of c-Myc expression at indicated time-points after infection with SL7207-SCR (shaded bars) and SL7207-c-Myc (white bars)(G-SW480, H-HCT116 & I-MCF7). Significant reduction of c-Myc mRNA was observed at timepoints indicated, with c-Myc mRNA levels normalised to Gapdh, Hsp909a and ActB mRNAs (t-test pairwise comparisons with * = P < 0.01, ** = P < 0.001 and *** = P < 0.0001). (J-L) Western analysis of protein samples from untreated cells (U), cells infected with SL7207/scr (S), and SL7207/c-Myc (M) demonstrating a specific semi-quantitative reduction in the abundance of c-Myc (J-SW480, K-HCT116 & L-MCF7)