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. 2024 Mar 20;17:1823–1837. doi: 10.2147/JIR.S455699

Figure 4.

Figure 4

ST36 injection of collagenase partly antagonizes the analgesic effect of acupuncture, and the expression of TRPA1 protein decreases. (A) The effect of collagenase injection on PWL enhancement of CFA mice by acupuncture (n = 6). (B) The effect of collagenase injection on PWT enhancement of CFA mice by acupuncture (n = 6, CFA vs control, ##p < 0.01; ACU vs CFA, *p < 0.05, **p < 0.01; ACU + collagenase vs ACU, ^p < 0.05, ^^p < 0.01). (C) Double-labeling immunofluorescence of TRPA1 (green) and mast cells (red, tryptase marker), DAPI (blue), scale bar = 50 µm. (D) The quantification of the ST36 TRPA1 protein expression by immunofluorescence (n = 4). (E) Immunofluorescence quantification of ST36 mast cells (n = 4). (F) Immunofluorescence quantitative analysis of ST36 TRPA1-positive mast cells (n = 4). (G)Double-labeling immunofluorescence of TRPA1 (green) and macrophages (red, CD68 marker), scale bar =5 0µm. (H) The quantification of ST36 macrophages via immunofluorescence (n = 4). (I) Immunofluorescence quantitative analysis of ST36 TRPA1-positive macrophages (n = 4). (J) Double-labelling immunofluorescence of TRPA1 (green) and fibroblasts (red, vimentin marker), scale bar = 50 µm. (K) Immunofluorescence quantitative analysis of ST36 fibroblasts (n = 4). (L) Immunofluorescence quantitative analysis of ST36 TRPA1-positive fibroblasts (n = 4, ACU vs ACU + collagenase, *p < 0.05, **p < 0.01).