TABLE 2.
Laboratory findingsa
| Animal no. | VIb | IFA/IPc | PCR specific ford:
|
EHDV-specific AGID | EHDV-2-specific RIPA | EHDV-2-specific RT in situ PCR | ||
|---|---|---|---|---|---|---|---|---|
| EHDV-1 | EHDV-2 | BTV | ||||||
| 1 | + | + | − | + | − | − | + | + |
| 2 | − | + | − | + | − | + | + | + |
| 3 | − | + | − | + | − | − | + | + |
| 4 | + | + | − | + | − | − | + | + |
| 5 | + | + | − | + | − | − | + | + |
| 6 | + | + | − | + | − | − | + | + |
| 7 | + | + | − | + | − | − | + | + |
| 8 | − | − | − | − | − | − | − | − |
| 9 | − | − | − | − | − | − | − | − |
| 10 | − | − | − | − | − | − | − | − |
a
Animals 8 to 10 represent clinically healthy, lesion-free deer and antelope from the affected area. Antiserum to BTV was cross-reactive with BTV serotypes 2, 10, 11, 13, 17, and 20.
b
VI, virus isolation. Erythrocyte lysates and/or tissue homogenates were inoculated into embryonated chicken eggs and/or CPAE cells.
c
IFA/IP, indirect immunofluorescence (IFA) and/or immunoperoxidase (IP) stain. Fluorescein- or biotin-conjugated MAbs specific for EHDV (hybridoma 11C6.288) or BTV (hybridoma 1004.90) inner capsid protein (VP7) were reacted with CPAE cells which had been cocultivated with clinical samples. MAbs were provided as a gift by Michael Jochim, Veterinary Diagnostic Technology, Inc., Wheat Ridge, Colo.