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Experimental workflow of SMOAC strategy for Phosphopeptide enrichment of fractionated tryptic peptides. Phosphopeptides are first enriched using Titanium dioxide (TiO2) chromatography. The eluent is collected and vacuum dried for MS/MS analysis. The wash fractions and flow through from TiO2 are pooled together and subjected to a second enrichment with Fe-NTA (Ferric nitrilo triacetate), the eluent from which is collected separately for MS/MS analysis.
