FIG. 2.

To identify the component of the disulfide aggregate presented in Fig. 1A, the 78-kDa and NSP4 bands from the gel presented in Fig. 1A (TM+BFA, 60-min chase) were excised and incubated with elution buffer (10 mM Tris-HCl [pH 7], 0.1% SDS) for 2 h at 37°C. The eluted 78-kDa protein was analyzed by nonreducing (lane b) and reducing (lane c) SDS-PAGE. The eluted NSP4 protein was analyzed by nonreducing SDS-PAGE (lane a). The lysate from the pulse-chase experiment presented in Fig. 1A (TM+BFA, 60-min chase) was immunoprecipitated with a VP7 MAb (M60) (lane d). M, molecular mass markers. The molecular masses (kilodaltons) of the markers are shown in the middle of the panel.