Figure 1.

Subcutaneous infection with S. aureus does not induce anti-SpA_mutIgG in mice. Anti-SpA_mut IgG titration expressed as relative light units (RLU) per milliliter in sera of animals only immunized, only infected,or immunized and infected. Pre-immune (Day 0, D0), post II (D52), or post-infection (D72) sera were analyzed and titers against SpA_mut were reported in the graph. Sham animals received only buffer, while SpA_mut/AS01 animals were immunized with the proposed vaccine. The median value and the 95% confidence interval were reported for each group and condition. Groups were represented by at least 7 animals (range, 7–11 animals/group). LLOQ, lower limit of quantification; ULOQ, upper limit of quantification. n.s. p _val > 0.05. The Kruskal–Wallis and uncorrected Dunn’s posttest was used to assess significance.