Figure 4.

Vaccination with SpA_mut/AS01 expands the antibody repertoire following an S. aureus infection. Nine single sera obtained from immunized and infected (SpA_mut/AS01 and infected) and from infected-only animals (sham and infected) were assessed against the staphylococcal protein microarray. Out of the 96 antigens spotted on the array, 23 reacted with higher frequency with SpA_mut/AS01 and infected sera, with respect to sera obtained from infected-only animals. Filled box: reactive serum; non-filled box: non-reactive serum. GAG L: Glycosaminoglycan lyase; RDG-lipop.: RDG lipoprotein; Thermon: thermonuclease. *p _val< 0.05; **p _val< 0.01; ***p _val< 0.001; ****p _val< 0.0001. Fisher’s exact t-test was used to evaluate significant differences between recognition frequencies for each antigen.