FIG. 2.

Titration of the 11-kDa promoter with Ni-agarose-purified protein. ³²P-labeled 120-nucleotide 11-kDa promoter DNA was mixed with 0 (lane 1), 100 (lanes 2 and 3), 200 (lane 4), 300 (lane 5), 400 (lane 6), 500 (lane 7), and 600 ng (lane 8) of protein in the presence of 40 ng of poly(dI-dC). The mobilities of complexes 1, 2, and 3 are indicated. The reaction corresponding to lane 3 included 1 mM MgCl₂ in addition to the standard binding buffer.