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DNase I footprinting of protein bound to the I1L promoter. I1L promoter 120-nucleotide DNA uniquely labeled on the template (A) or nontemplate (B) strand was treated with DNase I in the absence (lanes 1) or presence (lanes 2) of glycerol gradient-purified LPBP. Cleavage products were resolved on a 6% polyacrylamide DNA sequencing gel. Lanes 3 contain probe DNA chemically cleaved at purine residues. The nucleotide sequence of each strand of the I1L promoter is shown on the right.
