FIG. 4.

Infection time course of TR3, TR5/6, TR6, TR9, and TR8a/9 transcripts, determined by Northern blot analysis of RNA samples isolated at various times p.i. from CCO cells infected with CCV. Times indicated are measured from the start of infection. Cells were incubated with virus for 30 min, and samples were harvested at various intervals which ranged from 30 min after the PBS wash (the 1-h time point) to 4 h after the end of the infection period (4.5-h time point). Lanes: m.i., total RNA isolated from mock-infected CCO cells; c, total RNA isolated from CCV infected CCO cells harvested at 1, 1.5, 2, 2.5, 3.5, or 4.5 h after the initiation of infection either in the absence or presence (C) of cycloheximide. (A) Blot probed with a labeled cDNA encoding TR3; (B) duplicate blot probed with a labeled cDNA encoding TR5/6; (C) duplicate blot probed with a labeled cloned genomic fragment of 997 bp common to both TR8a/9 and TR9 transcripts. Arrows (right) mark the positions of the TR3, TR5/6, TR6, TR8a/9, and TR9 transcripts.