TABLE 1.

Placement and 2-base extension of tRNA₃^Lys within wild-type and mutant HIV-1 viruses

Virus	Genomic placement (% of wild type)a	2-base extension (% of tRNA3Lys placed)b
Wild type	100	80 ± 3
Mutants
R7R10K11 S	24 ± 5	60 ± 2
C15S/C18S	54 ± 8	40 ± 2
A30P	61 ± 9	83 ± 3
P31L	7 ± 4	NDc
R32G	19 ± 8	98 ± 3
S3(32-34)	25 ± 9	91 ± 2
C36S	94 ± 10	65 ± 2
C36S/C39S	105 ± 10	55 ± 2
K59L	87 ± 9	79 ± 2
Dr2	5 ± 2	ND
Cotransfection with pSVGag-RRE
R7R10K11 S	20 ± 5
P31L	5 ± 2
R32G	12 ± 6
S3(32-34)	17 ± 5
Cotransfection with pSVFS5TproD25G
R7R10K11 S	22 ± 5
P31L	120 ± 12
R32G	10 ± 5
S3(32-34)	20 ± 6

^aResults are means and standard deviations for single transfection experiments performed three times and cotransfection experiments performed two times. 

^bPercentage of total tRNA₃^Lys placed = [2-base extended/(unextended + 2-base extended) × 100. Results are means and standard deviations for experiments performed three times. 

^cND, not detected.