Table 2.
Evaluation of dilutions using the Roche Myoglobin assay on the cobas e602 analyzer
| Expected concentration (nmol/L) | Result of diluted specimen* (nmol/L) | Dilution | Final reported result* (nmol/L) | % Recovery |
|---|---|---|---|---|
| 48,744.33 | 121.86 | 1:400 | 48,744.33 | 100.0 |
| 24,375.28 | 68.00 | 1:400 | 27,201.07 | 111.6 |
| 12,190.73 | 35.86 | 1:400 | 14,342.58 | 117.7 |
| 6,098.48 | 132.19 | 1:50 | 6,609.66 | 108.4 |
| 3,052.36 | 67.31 | 1:50 | 3,365.67 | 110.3 |
| 1,529.27 | 37.29 | 1:50 | 1,864.61 | 121.9 |
| 767.75 | 84.94 | 1:10 | 849.44 | 110.6 |
| 386.97 | 44.03 | 1:10 | 440.34 | 113.8 |
| 196.57 | 23.51 | 1:10 | 235.11 | 119.6 |
| 101.40 | 12.90 | 1:10 | 129.04 | 127.3 |
| 53.82 | 6.75 | 1:10 | 67.53 | 125.5 |
| 30.00 | 4.06 | 1:10 | 40.56 | 135.2 |
| 18.09 | Not applicable | No dilution | 18.93 | 104.6 |
| 12.13 | Not applicable | No dilution | 12.87 | 106.1 |
| 9.16 | Not applicable | No dilution | 9.61 | 104.9 |
| 7.70 | Not applicable | No dilution | 7.87 | 102.2 |
| 6.97 | Not applicable | No dilution | 7.08 | 101.6 |
*The result of the diluted specimen is calculated by dividing the final reported result divided by the dilution performed (e.g., if the final reported result were 500 nmol/L and the dilution performed was 1:10, the calculation would be 500 nmol/L/10=50 nmol/L).