TABLE 1.
Summary of E2 mutant phenotypes
| E2 protein | Transactivationa | DNA bindingb | Viral DNA replicationb | Growth inhibitionc |
|---|---|---|---|---|
| Wild-type E2 | ++ | + | ++ | ++ |
| E2amber | − | +d | − | − |
| Q15H | − | + | + | − |
| E39G | − | + | ++ | − |
| F87S | +/− | + | + | − |
| W92R | − | + | − | − |
| W99C | − | + | + | − |
| E105G | +; ++e | + | ++ | +/− |
| K339M | − | − | ND | − |
| C340R | − | − | ND | − |
| VP16E2R | +++ | + | − | − |
a
Level of transactivation compared to that by wild-type E2. Symbols: −, 0 to 10%; +/−, 10 to 40%; +, 40 to 80%; ++, 80 to 120%; +++, >120%.
b
Data for DNA binding and viral DNA replication are from references 27, 41, and 56. Symbols for DNA binding: −, no detectable binding; +, readily detectable binding. Symbols for DNA replication: −, <10% of wild-type levels; +, 10 to 50%; ++, >90%. ND, not done.
c
Fold growth inhibition in infected HeLa cells relative to that in mock-infected cells. Symbols: −, zero to twofold; +/−, two- to fourfold; ++, >10-fold.
d
Activity of the E2-TR protein which is expressed by this mutan.
e
E105G scored + in HeLa cells and ++ in HT-3 cells.