FIG. 5.

Deletion mutation analysis of the effects of P62 on AT3 cell death. On top, a schematic of wild-type P62 is shown. Below, schematics of P62 deletion mutants are shown. ECD, E2 extracellular domain; CD, E2 cytoplasmic domain. The percentage loss of blue cells is relative to results achieved by transfection with the control empty vector, GH52. The numbers shown represent means ± standard errors of the mean for transfections done in triplicate from a representative experiment. Similar results were obtained in more than five independent transfections.