KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| Guinea-pig anti-insulin | Dako | Cat#A0564; RRID:AB_10013624 |
| Mouse anti-somatostatin | Santa Cruz Biotechnology | Cat#sc-55565; RRID:AB_831726 |
| Rabbit anti-TALK-1 | Novus Biologicals | Cat#NBP1-83071; RRID:AB_11021713 |
| Rabbit anti-TALK-1 | Alomone Labs | Cat#APC-170; RRID:AB_2756754 |
| Rabbit anti-Ki67 | Abcam | Cat#ab15580; RRID:AB_443209 |
| Rabbit anti-ALDH1A3 | Novus Biologicals | Cat#NBP2-15339; RRID:AB_2665496 |
| Mouse anti-8OHdG | Abcam | Cat#ab62623; RRID:AB_940049 |
| Mouse anti-FLAG M2 | Sigma Aldrich | Cat#F3165; RRID:AB_259529 |
| Rabbit anti-VDAC | Cell Signaling Technology | Cat#4661; RRID:AB_10557420 |
| Rabbit anti-calreticulin | Cell Signaling Technology | Cat#12238; RRID:AB_2688013 |
| Mouse anti-GAPDH | Thermo Fisher Scientific | Cat# MA5-15738; RRID:AB_10977387 |
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| Chemicals, peptides, and recombinant proteins | ||
|
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| Collagenase P | Roche | Cat#11249002001 |
| Histopaque | Sigma | Cat#10771 |
| HBSS | Invitrogen | Cat#14185 |
| RPMI | Invitrogen | Cat#11875 |
| DMEM | Invitrogen | Cat# 10569044 |
| Bovine Serum Albumin | Sigma | Cat#A8806 |
| Fetal Bovine Serum | Invitrogen | Cat#26140 |
| HEPES | Sigma | Cat#h3375 |
| Magnesium chloride hexahydrate | Fisher | Cat#AC41341-5000 |
| Magnesium sulfate heptahydrate | Sigma | Cat#63140 |
| Potassium chloride | Sigma | Cat#p4504 |
| Potassium phosphate monobasic | Sigma | Cat#60218 |
| Sodium chloride | Sigma | Cat#s7653 |
| Sodium phosphate dibasic dihydrate | Sigma | Cat#7907 |
| Tolbutamide | MP biomedicals | Cat#190274 |
| Triton X-100 non-ionic | Roache | Cat#10789704001 |
| Trypsin, 0.05% (1X) with EDTA 4Na | Invitrogen | Cat#25300 |
| Versene | Invitrogen | Cat#15040 |
| Adenosine 5’-triphosphate magnesium salt | Sigma | Cat#A9187 |
| Amphotericin B solubilized. | Sigma | Cat#a9528 |
| LB Broth | Sigma | Cat#l3022 |
| Fura-2,AM | Molecular Probes | Cat#F1221 |
| Rhodamine 123 | Thermo Fisher Scientific | Cat#R302 |
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| Critical commercial assays | ||
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| Agilent Seahorse XF Cell Mito Stress Test Kit | Agilent Technologies | Cat#103015-100 |
| Ultrasensitive Mouse Insulin ELISA | Mercodia Inc | Cat#10-1249-01 |
| Mouse Insulin ELISA | Alpco Ltd | 80-INSMS-E01 |
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| Experimental models: Cell lines | ||
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| 293FT Cell Line | Thermo Fisher Scientific | Cat#R70007 |
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| Experimental models: Organisms/strains | ||
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| Targeted ES cells-Kcnk16tm1a(EUCOMM)Hmgu were used to generate the Kcnk16 fl/fl mouse model at Vanderbilt University | EUCOMM IMPC | https://www.mousephenotype.org/data/alleles/MGI:1921821/tm1a(EUCOMM)Hmgu/ |
| Ins1 Cre mice; B6(Cg)-Ins1tm1.1(cre)Thor/J | (Thorens et al.)55; Obtained from the Jackson Laboratory | Strain #026801 |
| C57Bl/6J mice | The Jackson Laboratory | Strain #:000664 |
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| Recombinant DNA | ||
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| pMD2.G | a gift from Didier Trono, obtained from Addgene |
Cat #12259 |
| psPAX2 | a gift from Didier Trono, obtained from Addgene | Cat #12260 |
| RIP-Cepia2mt | (Suzuki et al.)30; Obtained from Addgene | Cat #58218 |
| RIP-4mtD3mCerulean3-16 | (Greotti et al.)34 | |
| CMV-PercevalHR | This paper and Addgene | Cat #49082 |
| pcDNA3.1-TALK-1 T3-FLAG | This paper | |
| pcDNA3.1-3XHA-EGFP-OMP25 | This paper and Addgene | Cat#83356 |
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| Software and algorithms | ||
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| GraphPad Prism v9.0.0 | Graphpad Software | https://www.graphpad.com/ |
| Clampfit 10.7 Software | Molecular Devices | https://www.moleculardevices.com/products/axon-patch-clamp-system |
| Biorender | Biorender | https://www.biorender.com/ |
| Nikon NIS-Elements | Nikon Healthcare | https://www.microscope.healthcare.nikon.com/ |