TABLE 1.
Physical properties of triplexes and triplex proteins
| Protein | Distance from meniscus (cm) | S | Experimentally determined mol wt | Actual mol wt from DNA sequence |
|---|---|---|---|---|
| BSAa | 1.9 | 4.7 | 68,000 | 68,000 |
| VP19C | 1.5 | 3.7 | 48,000 | 50,260 |
| VP23 | 2.0 | 4.9 | 72,000 | 34,268 |
| VP19C-VP23 | 2.9 | 7.2 | 129,000 | 118,796b |
BSA was used as a standard to calculate values for other proteins by using the Martin and Ames equation (17).
Molecular weight was calculated for a VP19C-VP232 heterotrimer.