TABLE 2.
Physical properties of truncated triplex proteins and protein complexes
| Protein | Distance from meniscus (cm) | S | Experimentally determined mol wt | Actual mol wt from DNA sequence |
|---|---|---|---|---|
| BSAa | 2.0 | 4.7 | 68,000 | 68,000 |
| cd15 | 1.5 | 3.5 | 44,000 | 48,640 |
| nd105 | 1.4 | 3.3 | 40,000 | 38,920 |
| cd15-VP23 | 3.0 | 7.1 | 126,000 | 117,176b |
| nd105-VP23 | 2.5 | 5.9 | 96,000 | 107,456b |
BSA was used as a standard to determine other values as described in the text.
Molecular weight was calculated for a heterotrimer (VP19C mutant-VP232).