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. 2024 Jan 23;149(7):2180–2189. doi: 10.1039/d3an01969a

Suggested starting parameters for the P-2000 laser puller with dual barrel, Pt wire electrodes.

Step Heat Filament Velocity Delay Pull
Seal 750 5 100 200 0
30 s ON/OFF ×4 cycles, flip capillary, ×4 cycles
Pull 725 3 120 128 250
Heat on time ∼5 s