TABLE 1.
Transcription efficiencies of 83-nt, unmodified tRNAPhe, and 3′ hairpin mutantsa
| Template | Relative efficiency (%)b |
|---|---|
| Wild type 28 nt | 100 |
| 83 nt | 90 |
| tRNAPhe (unmodified) | 50 |
| G+20-A | 46 |
| G+20-G | 45 |
| G+20-U | 31 |
| G+20-C | 8 |
| Tetra-A | 58 |
| Tetra-G | 108 |
| Tetra-U | 61 |
| Tetra-C | 33 |
| G+21 | 10 |
| GG+21 | 2 |
| -CCA | 100 |
| -CC | 18 |
| -C | 6 |
| -CG | 6 |
| -GC | 2 |
| G+20(+)-A | 51 |
| G+20(+)-G | 249 |
| Tetra-G(+) | 50 |
| Tetra-G(−) | 62 |
| Tetra-G-inv | 94 |
a
The incorporation of [32P]UMP was determined by Cerenkov counting of the radioactivity in reaction products in the gel and was corrected for the various numbers of UMP residues present in the negative-sense reaction product.
b
Compared to that of the 28-nt fragment. Results are averages for two experiments, except for the G+20 and tetra templates, which are averages for four experiments. Errors are about 5%.