TABLE 2.

Transcription efficiencies of RNAs unrelated to the 3′ end of TYMV RNA^a

Fragment	Relative efficiency (%)b
Wild type 44 nt	100
SSa	293
SSa(+)	102
SSc	264
hp-16S	14
PMPK5	2

^aThe incorporation of [³²P]UMP was determined by Cerenkov counting of the radioactivity in the reaction products in the gel and was corrected for the various numbers of UMP residues present in the negative-sense reaction product, assuming that a full-length transcript is synthesized. 

^bCompared to that of the 44-nt fragment. Results are averages for two experiments. Errors are about 5%.