TABLE 3.
Sizes of the double-stranded products of SSa, SSa(+), and SSc
| Template | Migration distance (cm) | bp | Relative efficiency (%)a |
|---|---|---|---|
| 44 nt | 3.70 | 43 | 100 |
| G+30 | 4.60 | 30 | |
| 28 nt | 5.00 | 27 | |
| Tetra-A | 7.00 | 18 | |
| SSa/SSa(+) | |||
| 1 | 3.70 | 42–46d | NDe/42 |
| 2 | 4.00 | 37 | ND/34 |
| 3b | 4.30 | 33 | 67d/0 |
| 4 | 4.85 | 29 | 54/4 |
| 5 | 5.45 | 25 | 109/8 |
| 6 | 6.10 | 22 | 73/16 |
| 7 | 6.90 | 19 | 70/81 |
| 8 | 8.50 | 13c | 16/1 |
| 9 | 9.00 | 11c | 19/9 |
| SSc | |||
| 1 | 3.70 | 41 | 67 |
| 2 | 4.00 | 37 | 34d |
| 3 | 4.30 | 33 | 30d |
| 4 | 4.60 | 30 | 73 |
| 5 | 5.00 | 27 | 15 |
| 6 | 5.50 | 24 | 8 |
| 7 | 6.15 | 22 | 6 |
| 8 | 7.00 | 18 | 9 |
| 9 | 8.70 | 13c | 35 |
a
Compared to that of the 44-nt fragment.
b
Not detected for SSa(+).
c
Hard to determine because of the influence of the nucleotide composition on the migration of the product.
d
Hard to determine because of poor resolution.
e
ND, not determined because of poor resolution.