REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
Lysogeny broth (LB) medium	Prepared by the media kitchen, Department of Biochemistry University of Oxford	N/A
M9 medium	Prepared by the media kitchen, Department of Biochemistry University of Oxford	N/A
Nematode growth medium (NGM) agar	Prepared by the media kitchen, Department of Biochemistry University of Oxford	N/A
Triton X-100	Sigma-Aldrich	CAS Number: 9036-19-5
Critical commercial assays
DNeasy Blood and Tissue Kit	Qiagen	Catalog no. 69504
Deposited data
Whole genome resequencing data of ancestral and evolved pathogen populations	National Center for Biotechnology Information (NCBI)	BioProject: PRJNA998467
Phenotypic and processed genomic data	Mendeley Data	Mendeley Data: https://doi.org/10.17632/xz9t9gjtw6.1
Experimental models: Organisms/strains
Organism: Caenorhabditis elegans strain N2 Bristol	Caenorhabditis Genetics Center	N2
Organism: Caenorhabditis elegans strain pmk-1 (M03F8.4(op497), pmk-1(km25))	Jonathan Hodgkin (University of Oxford)	Km25
Organism: Escherichia coli OP50	Caenorhabditis Genetics Center	OP50
Organism: Pseudomonas aeruginosa PA14-GFP	Kevin Foster (University of Oxford)	PA14
Organism: Pseudomonas berkeleyensis MSPm1	Michael Shapira (University of California at Berkeley)	MSPm1
Software and algorithms
R version 4.2.0	https://www.r-project.org	N/A
Breseq	https://barricklab.org/twiki/pub/Lab/ToolsBacterialGenomeResequencing/documentation/	N/A
Popoolation2	https://sourceforge.net/p/popoolation2/wiki/Home/	N/A
PHYLIP	https://phylipweb.github.io/phylip/	N/A