TABLE 1.
PBMC panels markers. Cell-surface markers used to gate and identify indicated cell types in PBMC samples
| Major cell population | Subsets | Gating | Reference |
|---|---|---|---|
| T/B Panel | |||
| Total T cells | CD45+/CD3+ | PMID: 32830910 | |
| CD8+ T cells | CD45+/CD3+/CD4−/CD8+ | PMID: 32830910 | |
| Naïve CD8 | CD45RA+/CD197+ | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| TEMRA CD8 | CD45RA+/CD197− | PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| CD45RA terminal effector CD8 | CD45RA+/CD197−/CD27−/CD28− | PMID: 18657274 | |
| Central memory CD8 | CD45RA−/CD197+ | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| Effector CD8 | CD45RA−/CD197− | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| Early effector CD8 | CD45RA−/CD197−/CD28+/CD27+ | PMID: 18785267; PMID: 18657274 | |
| Early-like effector CD8 | CD45RA−/CD197−/CD28+/CD27− | PMID: 18785267; PMID: 18657274 | |
| Terminal effector CD8 | CD45RA−/CD197−/CD28−/CD27− | PMID: 18785267; PMID: 18657274 | |
| Intermediate effector CD8 | CD45RA−/CD197−/CD28−/CD27+ | PMID: 18785267; PMID: 18657274 | |
| CD4+ T cells | CD45+/CD3+/CD8 − /CD4+ | PMID: 32830910 | |
| Naïve CD4 | CD45RA+/CD197+ | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| TEMRA CD4 | CD45RA+/CD197− | PMID: 10537110; PMID: 28069807; PMID: 18657274; PMID: 29133794 | |
| CD45RA terminal effector CD4 | CD45RA+/CD197−/CD27−/CD28− | PMID: 18657274; PMID: 29133794 | |
| Central memory CD4 | CD45RA−/CD197+ | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| Follicular helper T | CD45RA−/CD197+/CD185+/CD183all | PMID: 30714682 | |
| Effector CD4 | CD45RA−/CD197− | PMID: 24258910; PMID: 10537110; PMID: 28069807; PMID: 18657274 | |
| Early effector CD4 | CD45RA−/CD197−/CD28+/CD27+ | PMID: 18785267; PMID: 18657274 | |
| Early-like effector CD4 | CD45RA−/CD197−/CD28+/CD27− | PMID: 18785267; PMID: 18657274 | |
| Terminal effector CD4 | CD45RA−/CD197−/CD28−/CD27− | PMID: 18785267; PMID: 18657274 | |
| Intermediate effector CD4 | CD45RA−/CD197−/CD28−/CD27+ | PMID: 18785267; PMID: 18657274 | |
| Regulatory T cells | CD25+/CD127− | PMID: 23166007 | |
| Th1 | CD183+/CD196− | PMID: 33219622; PMID: 30714682 | |
| Th2 | CD194+/CD196− | PMID: 33219622; PMID: 30714682 | |
| Th9/22 | CD183−/CD196+/CD194+ | PMID: 30714682 | |
| Th17 | CD161+/CD196+ | PMID: 30714682 | |
| Gamma delta T cells | CD45+/CD3+/TCRγδ+ | PMID: 28119690; PMID: 32830910 | |
| CD197−/CD45RA++/TCRgd+ | CD197−/CD45RA++ | PMID: 12900516; PMID: 32830910 | |
| CD197+/CD45RAall/TCRgd+ | CD197+/CD45RAall | PMID: 12900516; PMID: 32830910 | |
| CD197−/CD45RA−/TCRgd+ | CD197−/CD45RA− | PMID: 12900516; PMID: 32830910 | |
| Activated T cells | CD45+/CD3+/HLA−DR+/CD38+ | PMID: 26362266; PMID: 29483513 | |
| NKT-like T cells | CD45+/CD3+/TCRγδ−/CD161+ | PMID: 21787777 | |
| NK cells | CD45+/CD3−/TCRγδ−/TCRαβ−/HLA-DR − | PMID: 32830910 | |
| Total B cells | CD45+/CD19+ | PMID: 28715846 | |
| Plasmablasts | CD27+/CD38+ | PMID: 28715846; PMID: 15778361 | |
| CD20+ B cells | CD20+ | PMID: 28715846 | |
| Naïve | CD20+/CD27−/IgD+ | PMID: 28715846; PMID: 9802980 | |
| Switched memory B | CD20+/CD27+/IgD− | PMID: 28715846; PMID: 9802980 | |
| Unswitched memory B | CD20+/CD27+/IgD+ | PMID: 28715846; PMID: 9802980 | |
| DN memory B | CD20+/CD27−/IgD− | PMID: 28715846; PMID: 9802980 | |
| Checkpoint inhibitors | PD-1 | CD279+ | PMID: 32429929 |
| TIM-3 | CD366+ | PMID: 32429929 | |
| LAG-3 | CD223+ | PMID: 32429929 | |
| CTLA-4 | CD152+ | PMID: 32429929 | |
| M/N/D Panel | |||
| NKT cells | CD45+/CD3+/CD56+ | PMID: 24779018 | |
| NK cells | CD45+/CD3−/CD19−/CD14−/CD7+ | PMID: 19805616 | |
| Early NK cells | CD56+/CD16− | PMID: 23650273; PMID: 32830910 | |
| Mature NK cells | CD56+/CD16+ | PMID: 23650273; PMID: 32830910 | |
| Terminal NK cells | CD56−/CD16+ | PMID: 23650273; PMID: 32830910 | |
| NK cell status | KLRD1 | CD94+ | PMID: 23650273 |
| NKG2D | CD314+ | PMID: 23650273 | |
| NKG2C | CD159c+ | PMID: 23650273 | |
| NKp30 | CD337+ | PMID: 23650273 | |
| NKp46 | CD335+ | PMID: 23650273 | |
| NKp44 | CD336+ | PMID: 23650273 | |
| KIR2DL1/S1/S3/S5 | CD158+ | PMID: 23650273 | |
| TIM-3 | CD366+ | PMID: 32858904 | |
| Classical monocytes | CD45+/CD14+/CD16− | PMID: 20628149 | |
| Classical monocytes-Normal | HLA-DR+ | PMID: 31191529 | |
| Classical monocytes-Immunosuppressed | HLA-DR− | PMID: 31191529 | |
| Intermediate monocytes | CD45+/CD14+/CD16+ | PMID: 20628149 | |
| Intermediate monocytes-Normal | HLA-DR+ | PMID: 31191529 | |
| Intermediate monocytes-Immunosuppressed | HLA-DR− | PMID: 31191529 | |
| Nonclassical monocytes | CD45+/CD14− /CD16+ | PMID: 20628149 | |
| Nonclassical monocytes-Normal | HLA-DR+ | PMID: 31191529 | |
| Nonclassical monocytes-Immunosuppressed | HLA-DR− | PMID: 31191529 | |
| MDSC-like cells | CD45+/CD11b+/CD33+/HLA-DR− | PMID: 28936253 | |
| Monocytic MDSC-like cells | CD14+ | PMID: 28936253 | |
| Dendritic cells | CD45+/CD3−/HLA-DR+/CD19−/CD20− | PMID: 29356334 | |
| cDC1 | CD14−/CD123−/CD141+/CD1c−/CD5− | PMID: 29356334, PMID: 31474513 | |
| cDC2 | CD14−/CD123−/CD141−/CD1c+/CD5+/ CD11b+ | PMID: 29356334, PMID: 31474513 | |
| cDC3 | CD14±/ CD163±/CD5− | PMID: 28428369; PMID: 31474513 | |
| pDC | CD14−/CD123+/CD141−/CD1c−/CD5− | PMID: 29356334; PMID: 28167780 | |