TABLE 4.
Quadruple verification analysis of SV40 regulatory region sequences of clones recovered from monkey tissues
| Animal | Prototype virus clonea | No. of infectious virus isolates:
|
Result by viral DNA detection
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Recovered from filtratesb
|
Recovered from DNA lipofectionsb
|
PCRc
|
LPCRd
|
||||||||
| Brain | Spleen | Brain | PBMC | Spleen | Brain | PBMC | Spleen | Brain | Spleen | ||
| 6593 | SV40-6593-1 | 12 | NDe | 4 | 2 | ND | + | + | ND | 4 | ND |
| SV40-6593-2 | 2 | ND | 1 | 1 | ND | + | + | ND | 1 | ND | |
| SV40-6593-5 | 4 | ND | 1 | 1 | ND | + | + | ND | 1 | ND | |
| H328 | SV40-H328-1 | 6 | ND | 2 | 1 | ND | + | + | ND | 6 | ND |
| SV40-H328-2 | 0 | ND | 0 | 1 | ND | 0 | + | ND | 0 | ND | |
| H491 | SV40-H491-1 | 8 | ND | 2 | 0 | ND | + | + | ND | 6 | ND |
| H388 | SV40-H388-1 | 12 | ND | 2 | 2 | ND | + | + | ND | 3 | ND |
| SV40-H388-2 | 2 | ND | 1 | 1 | ND | + | + | ND | 2 | ND | |
| SV40-H388-16 | 4 | ND | 1 | 1 | ND | + | + | ND | 2 | ND | |
| H822 | SV40-H822-1 | 8 | ND | 2 | ND | ND | + | ND | ND | 6 | ND |
| I508 | SV40-I508-1 | 8 | ND | 0 | 0 | ND | + | + | ND | 6 | ND |
| K661 | SV40-K661-1 | 8 | ND | 4 | 0 | ND | + | + | ND | 6 | ND |
| T302 | SV40-T302-1 | 8 | 5 | 2 | ND | 3 | + | ND | + | 2 | 1 |
a
See Fig. 2D for the structure of the regulatory region of each indicated virus clone.
b
Numbers are the numbers of individual clones verified by DNA sequencing to have identity with the prototype. Additional clones analyzed only by ethidium-bromide gel electrophoresis are not shown.
c
+, PCR product that comigrated with PCR product amplified from control template (PCR products were not sequenced); 0, no PCR product detected.
d
Values are numbers of clones analyzed for regulatory region by ethidium-bromide gel electrophoresis. Only the regulatory regions of SV40-H328, SV40-K661, and SV40-T302 LPCR clones were sequenced.
e
ND, not done.