Figure 4.

(A and B) k _on data from the lines of best fit in Figure 3C and D plotted using Eq. 6 to obtain limiting Ivosidenib $k_{\mathrm{on}}^0$ values (and K _d values) where [2OG] and [Mg²⁺] equal 0 M. (C) Plot showing how k _obs varies with the Ivosidenib concentration at two concentrations of Mg²⁺ ([2OG]=1 mM). The rate of inhibition is initially linear with increasing inhibitor concentrations but ultimately reaches a limiting value that is Mg²⁺ dependent. The grey rectangle re‐shows the data from Figure 3D. The k _obs values outside of the linear region were measured under the same experimental conditions as those described in Figure 3D (2OG=1 mM); each of these data points represents a single experiment. (D) Data from Figure 4C plotted according to Eq. 11 to obtain $k_{\mathrm{on}}^{\mathrm{trans}}$ and $K_{\mathrm{d}}^{\mathrm{pre}}$ values.