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. 2024 Mar 22;16(1):2331520. doi: 10.1080/19490976.2024.2331520

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© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 3. — Permeability of colon epithelial models and phage translocation across these models.

a. Trans-epithelial electrical resistance (TEER) was measured before phage treatment showing increased resistance in differentiated/mature monolayers. b. Following the phage translocation assay, FITC-dextran was used to measure the degree of permeability of cell monolayers by measuring FITC fluorescence intensity in the basolateral chamber media. c, d. Ec70 phage titre collected from the basolateral chamber at 2 h (c) and 24 h (d) after administration of EC70 (10⁷ PFU) to the transwell. Unpaired t-test, * p< .5, ** p< .01, ***p< .005. Diff-, undifferentiated, Diff+, differentiated.